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c terminal myc ddk tags  (OriGene)


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    OriGene c terminal myc ddk tags
    C Terminal Myc Ddk Tags, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+terminal+ddk/DDRGK1+(NM_023935)+Human+Tagged+ORF+Clone/pm42277217-175-20-26
    Average 94 stars, based on 1 article reviews
    c terminal myc ddk tags - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Novel p65 Binding Glucocorticoid-induced Leucine Zipper Peptide Suppresses Experimental Autoimmune Encephalomyelitis
    Article Snippet: .. Recombinant human p65 protein (r-p65) and purified r-GILZ with C-terminal DDK (catalog number TP320780) and biotinylated anti-DDK antibody were from OriGene Technologies Inc., Rockville, MD. .. Partial length p65 (p65ΔC14) and anti-p65 mAb were from Active Motif, Carlsbad, CA.

    Purification:

    Article Title: Novel p65 Binding Glucocorticoid-induced Leucine Zipper Peptide Suppresses Experimental Autoimmune Encephalomyelitis
    Article Snippet: .. Recombinant human p65 protein (r-p65) and purified r-GILZ with C-terminal DDK (catalog number TP320780) and biotinylated anti-DDK antibody were from OriGene Technologies Inc., Rockville, MD. .. Partial length p65 (p65ΔC14) and anti-p65 mAb were from Active Motif, Carlsbad, CA.



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    c terminally tagged myc ddk mrpl40  (OriGene)
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    A. Proteins of the SLC25A1 interactome according to Gokhale et al . Edges were defined by the proximity ligation mitochondrial interactome according to Antonicka et al. . B. <t>MRPL40</t> and SLC25A1 immunoblots of wild type and MRPL40 null cell lines. C. Immunoblot of respiratory chain proteins encoded by the mitochondrial genome. D. Immunoblot of mitochondrial ribosome subunits. All subunits, except for MRPL44, are depicted in the SLC25A1 interactome. E. Immunoblot of SLC25A1 interactome proteins involved in acetyl-CoA and lipid metabolism. F. Immunoblot of RNA binding proteins present in the SLC25A1 interactome. HSP90 and ACTB were used as controls for B-F. G . Quantification of blots in panels B-F. Depicted is the ratio between mutant and wild type. Each dot represents an independent clone and/or independent experiment. p values were obtained with unpaired mean difference two-sided permutation t-test (italicized numbers represent p values). H . Metabolic activity in wild type and MRPL40 KO cells measured by the Seahorse Mito Stress Test (n=8 of each genotype). Oxygen consumption and extracellular acidification rates, OCR and ECAR, data are presented normalized to protein and analyzed by unpaired mean difference two-sided permutation t-test (italicized numbers represent p values). Basal, ATP-dependent, and maximal respiration were determined as described. Arrows indicate the sequential addition of oligomycin, FCCP and rotenone-antimycin. I. qRT-PCR quantification of mitochondrial rRNAs RNR1-2 and vimentin as control. Unpaired mean difference two-sided permutation t-test, n=3. J . Nanostring quantification of nuclear encoded and mitochondrial encoded RNAs. Junctions represent non-processed intermediaries derived from the polycistronic mitochondrial RNA. p values two-sided t test. Wild type n=6 and KO n=3. K . Immunofluorescent microscopy of with type and MRPL40 KO cells labeled for TOM20 and dsRNA. Scale bars correspond to 10 and 2.5 µm. Probability plot depicts the levels of dsRNA signal in 16 wild type and 20 MRPL40 KO cells. Kolmogorov-Smirnov test. L . Quantification of the ratio of mitochondrial and nuclear genomes. Unpaired mean difference two-sided permutation t-test. Wild type n=16 and KO n=20 cells.
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    A. Proteins of the SLC25A1 interactome according to Gokhale et al . Edges were defined by the proximity ligation mitochondrial interactome according to Antonicka et al. . B. <t>MRPL40</t> and SLC25A1 immunoblots of wild type and MRPL40 null cell lines. C. Immunoblot of respiratory chain proteins encoded by the mitochondrial genome. D. Immunoblot of mitochondrial ribosome subunits. All subunits, except for MRPL44, are depicted in the SLC25A1 interactome. E. Immunoblot of SLC25A1 interactome proteins involved in acetyl-CoA and lipid metabolism. F. Immunoblot of RNA binding proteins present in the SLC25A1 interactome. HSP90 and ACTB were used as controls for B-F. G . Quantification of blots in panels B-F. Depicted is the ratio between mutant and wild type. Each dot represents an independent clone and/or independent experiment. p values were obtained with unpaired mean difference two-sided permutation t-test (italicized numbers represent p values). H . Metabolic activity in wild type and MRPL40 KO cells measured by the Seahorse Mito Stress Test (n=8 of each genotype). Oxygen consumption and extracellular acidification rates, OCR and ECAR, data are presented normalized to protein and analyzed by unpaired mean difference two-sided permutation t-test (italicized numbers represent p values). Basal, ATP-dependent, and maximal respiration were determined as described. Arrows indicate the sequential addition of oligomycin, FCCP and rotenone-antimycin. I. qRT-PCR quantification of mitochondrial rRNAs RNR1-2 and vimentin as control. Unpaired mean difference two-sided permutation t-test, n=3. J . Nanostring quantification of nuclear encoded and mitochondrial encoded RNAs. Junctions represent non-processed intermediaries derived from the polycistronic mitochondrial RNA. p values two-sided t test. Wild type n=6 and KO n=3. K . Immunofluorescent microscopy of with type and MRPL40 KO cells labeled for TOM20 and dsRNA. Scale bars correspond to 10 and 2.5 µm. Probability plot depicts the levels of dsRNA signal in 16 wild type and 20 MRPL40 KO cells. Kolmogorov-Smirnov test. L . Quantification of the ratio of mitochondrial and nuclear genomes. Unpaired mean difference two-sided permutation t-test. Wild type n=16 and KO n=20 cells.
    C Terminal Myc Ddk Tag Carrying Akt1, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    c terminal ddk tag  (OriGene)
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    A. Proteins of the SLC25A1 interactome according to Gokhale et al . Edges were defined by the proximity ligation mitochondrial interactome according to Antonicka et al. . B. <t>MRPL40</t> and SLC25A1 immunoblots of wild type and MRPL40 null cell lines. C. Immunoblot of respiratory chain proteins encoded by the mitochondrial genome. D. Immunoblot of mitochondrial ribosome subunits. All subunits, except for MRPL44, are depicted in the SLC25A1 interactome. E. Immunoblot of SLC25A1 interactome proteins involved in acetyl-CoA and lipid metabolism. F. Immunoblot of RNA binding proteins present in the SLC25A1 interactome. HSP90 and ACTB were used as controls for B-F. G . Quantification of blots in panels B-F. Depicted is the ratio between mutant and wild type. Each dot represents an independent clone and/or independent experiment. p values were obtained with unpaired mean difference two-sided permutation t-test (italicized numbers represent p values). H . Metabolic activity in wild type and MRPL40 KO cells measured by the Seahorse Mito Stress Test (n=8 of each genotype). Oxygen consumption and extracellular acidification rates, OCR and ECAR, data are presented normalized to protein and analyzed by unpaired mean difference two-sided permutation t-test (italicized numbers represent p values). Basal, ATP-dependent, and maximal respiration were determined as described. Arrows indicate the sequential addition of oligomycin, FCCP and rotenone-antimycin. I. qRT-PCR quantification of mitochondrial rRNAs RNR1-2 and vimentin as control. Unpaired mean difference two-sided permutation t-test, n=3. J . Nanostring quantification of nuclear encoded and mitochondrial encoded RNAs. Junctions represent non-processed intermediaries derived from the polycistronic mitochondrial RNA. p values two-sided t test. Wild type n=6 and KO n=3. K . Immunofluorescent microscopy of with type and MRPL40 KO cells labeled for TOM20 and dsRNA. Scale bars correspond to 10 and 2.5 µm. Probability plot depicts the levels of dsRNA signal in 16 wild type and 20 MRPL40 KO cells. Kolmogorov-Smirnov test. L . Quantification of the ratio of mitochondrial and nuclear genomes. Unpaired mean difference two-sided permutation t-test. Wild type n=16 and KO n=20 cells.
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    A. Proteins of the SLC25A1 interactome according to Gokhale et al . Edges were defined by the proximity ligation mitochondrial interactome according to Antonicka et al. . B. MRPL40 and SLC25A1 immunoblots of wild type and MRPL40 null cell lines. C. Immunoblot of respiratory chain proteins encoded by the mitochondrial genome. D. Immunoblot of mitochondrial ribosome subunits. All subunits, except for MRPL44, are depicted in the SLC25A1 interactome. E. Immunoblot of SLC25A1 interactome proteins involved in acetyl-CoA and lipid metabolism. F. Immunoblot of RNA binding proteins present in the SLC25A1 interactome. HSP90 and ACTB were used as controls for B-F. G . Quantification of blots in panels B-F. Depicted is the ratio between mutant and wild type. Each dot represents an independent clone and/or independent experiment. p values were obtained with unpaired mean difference two-sided permutation t-test (italicized numbers represent p values). H . Metabolic activity in wild type and MRPL40 KO cells measured by the Seahorse Mito Stress Test (n=8 of each genotype). Oxygen consumption and extracellular acidification rates, OCR and ECAR, data are presented normalized to protein and analyzed by unpaired mean difference two-sided permutation t-test (italicized numbers represent p values). Basal, ATP-dependent, and maximal respiration were determined as described. Arrows indicate the sequential addition of oligomycin, FCCP and rotenone-antimycin. I. qRT-PCR quantification of mitochondrial rRNAs RNR1-2 and vimentin as control. Unpaired mean difference two-sided permutation t-test, n=3. J . Nanostring quantification of nuclear encoded and mitochondrial encoded RNAs. Junctions represent non-processed intermediaries derived from the polycistronic mitochondrial RNA. p values two-sided t test. Wild type n=6 and KO n=3. K . Immunofluorescent microscopy of with type and MRPL40 KO cells labeled for TOM20 and dsRNA. Scale bars correspond to 10 and 2.5 µm. Probability plot depicts the levels of dsRNA signal in 16 wild type and 20 MRPL40 KO cells. Kolmogorov-Smirnov test. L . Quantification of the ratio of mitochondrial and nuclear genomes. Unpaired mean difference two-sided permutation t-test. Wild type n=16 and KO n=20 cells.

    Journal: bioRxiv

    Article Title: Suppressive Genetic Interactions Between Haploinsufficient Mitochondrial Genes Encoded in the 22q11.2 Microdeletion Locus Define Brain and Cardiac Phenotypes

    doi: 10.64898/2026.01.09.698677

    Figure Lengend Snippet: A. Proteins of the SLC25A1 interactome according to Gokhale et al . Edges were defined by the proximity ligation mitochondrial interactome according to Antonicka et al. . B. MRPL40 and SLC25A1 immunoblots of wild type and MRPL40 null cell lines. C. Immunoblot of respiratory chain proteins encoded by the mitochondrial genome. D. Immunoblot of mitochondrial ribosome subunits. All subunits, except for MRPL44, are depicted in the SLC25A1 interactome. E. Immunoblot of SLC25A1 interactome proteins involved in acetyl-CoA and lipid metabolism. F. Immunoblot of RNA binding proteins present in the SLC25A1 interactome. HSP90 and ACTB were used as controls for B-F. G . Quantification of blots in panels B-F. Depicted is the ratio between mutant and wild type. Each dot represents an independent clone and/or independent experiment. p values were obtained with unpaired mean difference two-sided permutation t-test (italicized numbers represent p values). H . Metabolic activity in wild type and MRPL40 KO cells measured by the Seahorse Mito Stress Test (n=8 of each genotype). Oxygen consumption and extracellular acidification rates, OCR and ECAR, data are presented normalized to protein and analyzed by unpaired mean difference two-sided permutation t-test (italicized numbers represent p values). Basal, ATP-dependent, and maximal respiration were determined as described. Arrows indicate the sequential addition of oligomycin, FCCP and rotenone-antimycin. I. qRT-PCR quantification of mitochondrial rRNAs RNR1-2 and vimentin as control. Unpaired mean difference two-sided permutation t-test, n=3. J . Nanostring quantification of nuclear encoded and mitochondrial encoded RNAs. Junctions represent non-processed intermediaries derived from the polycistronic mitochondrial RNA. p values two-sided t test. Wild type n=6 and KO n=3. K . Immunofluorescent microscopy of with type and MRPL40 KO cells labeled for TOM20 and dsRNA. Scale bars correspond to 10 and 2.5 µm. Probability plot depicts the levels of dsRNA signal in 16 wild type and 20 MRPL40 KO cells. Kolmogorov-Smirnov test. L . Quantification of the ratio of mitochondrial and nuclear genomes. Unpaired mean difference two-sided permutation t-test. Wild type n=16 and KO n=20 cells.

    Article Snippet: To generate stable cell lines, SH-SY5Y cells (ATCC, CRL-2266; RRID:CVCL_0019) were transfected with ORF expression clone containing C terminally tagged Myc-DDK MRPL40 (Origene, RC202166) or N terminally tagged FLAG-SLC25A1 (GeneCopoeia, EX-A1932-Lv1020GS) as described .

    Techniques: Ligation, Western Blot, RNA Binding Assay, Mutagenesis, Activity Assay, Quantitative RT-PCR, Control, Derivative Assay, Microscopy, Labeling

    A. MRPL40 and SLC25A1 coprecipitate. Extracts from neuroblastoma cells stably expressing either MRPL40-FLAG or SLC25A1-FLAG were immunoprecipitated with FLAG antibodies (lanes 2-3) in the absence or presence of an excess FLAG peptide (lane 3). Lane 1 corresponds to input. Asterisks mark antigens tested. Double asterisk marks endogenous SLC25A1 and single asterisk labels SLC25A1-FLAG. B. Sanger sequencing chromatograms of wild type and MRPL40 null clones (See ). Homozygotes and compound heterozygote clones are presented. Clone 8 has a deletion of 7bp and 1 bp insertion (-7/+1). C . Immunoblot of the respiratory chain protein MT-CO3, encoded by the mitochondrial genome, and the RNA processing factors GRSF1 and LRPPR encoded by the mitochondrial genome. Two-sided permutation t-tests. Each dot is a biological replicate of one MRPL40 null clone analyzed.

    Journal: bioRxiv

    Article Title: Suppressive Genetic Interactions Between Haploinsufficient Mitochondrial Genes Encoded in the 22q11.2 Microdeletion Locus Define Brain and Cardiac Phenotypes

    doi: 10.64898/2026.01.09.698677

    Figure Lengend Snippet: A. MRPL40 and SLC25A1 coprecipitate. Extracts from neuroblastoma cells stably expressing either MRPL40-FLAG or SLC25A1-FLAG were immunoprecipitated with FLAG antibodies (lanes 2-3) in the absence or presence of an excess FLAG peptide (lane 3). Lane 1 corresponds to input. Asterisks mark antigens tested. Double asterisk marks endogenous SLC25A1 and single asterisk labels SLC25A1-FLAG. B. Sanger sequencing chromatograms of wild type and MRPL40 null clones (See ). Homozygotes and compound heterozygote clones are presented. Clone 8 has a deletion of 7bp and 1 bp insertion (-7/+1). C . Immunoblot of the respiratory chain protein MT-CO3, encoded by the mitochondrial genome, and the RNA processing factors GRSF1 and LRPPR encoded by the mitochondrial genome. Two-sided permutation t-tests. Each dot is a biological replicate of one MRPL40 null clone analyzed.

    Article Snippet: To generate stable cell lines, SH-SY5Y cells (ATCC, CRL-2266; RRID:CVCL_0019) were transfected with ORF expression clone containing C terminally tagged Myc-DDK MRPL40 (Origene, RC202166) or N terminally tagged FLAG-SLC25A1 (GeneCopoeia, EX-A1932-Lv1020GS) as described .

    Techniques: Stable Transfection, Expressing, Immunoprecipitation, Sequencing, Clone Assay, Western Blot

    A. Coessentiality network analysis showing genome-scale fitness correlations of SLC25A1 and MRPL40 as inputs. Nodes correspond to first and second level interaction nodes. Network of correlated (red edges) and anticorrelated genes (blue edges) was built with the FIREWORKS tool. r= Pearson correlation. B . Subnetwork of correlated and anticorrelated genes linked to components of the SLC25A1 interactome (red font nodes). C . ENRICHR Gene ontologies inferred from first order nodes connected to either SLC25A1 or MRPL40. Red and blue fonts depict correlated and anticorrelated genes, respectively.

    Journal: bioRxiv

    Article Title: Suppressive Genetic Interactions Between Haploinsufficient Mitochondrial Genes Encoded in the 22q11.2 Microdeletion Locus Define Brain and Cardiac Phenotypes

    doi: 10.64898/2026.01.09.698677

    Figure Lengend Snippet: A. Coessentiality network analysis showing genome-scale fitness correlations of SLC25A1 and MRPL40 as inputs. Nodes correspond to first and second level interaction nodes. Network of correlated (red edges) and anticorrelated genes (blue edges) was built with the FIREWORKS tool. r= Pearson correlation. B . Subnetwork of correlated and anticorrelated genes linked to components of the SLC25A1 interactome (red font nodes). C . ENRICHR Gene ontologies inferred from first order nodes connected to either SLC25A1 or MRPL40. Red and blue fonts depict correlated and anticorrelated genes, respectively.

    Article Snippet: To generate stable cell lines, SH-SY5Y cells (ATCC, CRL-2266; RRID:CVCL_0019) were transfected with ORF expression clone containing C terminally tagged Myc-DDK MRPL40 (Origene, RC202166) or N terminally tagged FLAG-SLC25A1 (GeneCopoeia, EX-A1932-Lv1020GS) as described .

    Techniques:

    A. Estimation of mouse embryonic viability using DepMap gene dependency scores of human MRPL40, TFAM, POLG, and TYR. B . Experimental design to generate an Mrpl40 null allele. C . Mrpl40 genomic region, CRISPR mutagenesis strategy, founder mutation, and predicted protein truncation. D . Frequence of Mrpl40 genotypes at embryonic age 14.5 from timed Mrpl40 -/+ intercrosses. E . Diagram of Slc25a1 -/+ ; Mrpl40 -/+ timed intercrosses, frequency of genotypes, and crown-rump lengths of embryos at embryonic age 14.5. Chi square test of expected and observed genotypes.

    Journal: bioRxiv

    Article Title: Suppressive Genetic Interactions Between Haploinsufficient Mitochondrial Genes Encoded in the 22q11.2 Microdeletion Locus Define Brain and Cardiac Phenotypes

    doi: 10.64898/2026.01.09.698677

    Figure Lengend Snippet: A. Estimation of mouse embryonic viability using DepMap gene dependency scores of human MRPL40, TFAM, POLG, and TYR. B . Experimental design to generate an Mrpl40 null allele. C . Mrpl40 genomic region, CRISPR mutagenesis strategy, founder mutation, and predicted protein truncation. D . Frequence of Mrpl40 genotypes at embryonic age 14.5 from timed Mrpl40 -/+ intercrosses. E . Diagram of Slc25a1 -/+ ; Mrpl40 -/+ timed intercrosses, frequency of genotypes, and crown-rump lengths of embryos at embryonic age 14.5. Chi square test of expected and observed genotypes.

    Article Snippet: To generate stable cell lines, SH-SY5Y cells (ATCC, CRL-2266; RRID:CVCL_0019) were transfected with ORF expression clone containing C terminally tagged Myc-DDK MRPL40 (Origene, RC202166) or N terminally tagged FLAG-SLC25A1 (GeneCopoeia, EX-A1932-Lv1020GS) as described .

    Techniques: CRISPR, Mutagenesis

    A . Uniform Manifold Approximation and Projection (UMAP) of cortical transcriptome before and after one-way ANOVA thresholding by p<0.01 of the indicated genotype transcriptomes. B . PCA of transcriptomes after one-way ANOVA thresholding by p<0.01. Lines represent Euclidean distance clustering. C. Normalized mRNA counts for Mrpl40 and Slc25a1 transcripts. Kruskal-Wallis test followed by Benjamini and Hochberg multiple corrections. D . Volcano plots and E . heat maps of paired comparisons between wild type animals with single or transheterozygotic animals. Insets in volcano plots show percentage of diverse types of RNAs differentially expressed. Heat Maps depict z-scored hierarchical clustering of transcripts after thresholding with a cut-off fold of change=1.5 and p<0.01 (Welsh t test). F and G depict violin plots of mRNAs either rescued (F) or worsened (G) in Slc25a1 -/+ ; Mrpl40 -/+ cortex. Kruskal-Wallis test followed by Benjamini and Hochberg multiple corrections. All panels wild type n=9 and all other genotypes n=7.

    Journal: bioRxiv

    Article Title: Suppressive Genetic Interactions Between Haploinsufficient Mitochondrial Genes Encoded in the 22q11.2 Microdeletion Locus Define Brain and Cardiac Phenotypes

    doi: 10.64898/2026.01.09.698677

    Figure Lengend Snippet: A . Uniform Manifold Approximation and Projection (UMAP) of cortical transcriptome before and after one-way ANOVA thresholding by p<0.01 of the indicated genotype transcriptomes. B . PCA of transcriptomes after one-way ANOVA thresholding by p<0.01. Lines represent Euclidean distance clustering. C. Normalized mRNA counts for Mrpl40 and Slc25a1 transcripts. Kruskal-Wallis test followed by Benjamini and Hochberg multiple corrections. D . Volcano plots and E . heat maps of paired comparisons between wild type animals with single or transheterozygotic animals. Insets in volcano plots show percentage of diverse types of RNAs differentially expressed. Heat Maps depict z-scored hierarchical clustering of transcripts after thresholding with a cut-off fold of change=1.5 and p<0.01 (Welsh t test). F and G depict violin plots of mRNAs either rescued (F) or worsened (G) in Slc25a1 -/+ ; Mrpl40 -/+ cortex. Kruskal-Wallis test followed by Benjamini and Hochberg multiple corrections. All panels wild type n=9 and all other genotypes n=7.

    Article Snippet: To generate stable cell lines, SH-SY5Y cells (ATCC, CRL-2266; RRID:CVCL_0019) were transfected with ORF expression clone containing C terminally tagged Myc-DDK MRPL40 (Origene, RC202166) or N terminally tagged FLAG-SLC25A1 (GeneCopoeia, EX-A1932-Lv1020GS) as described .

    Techniques:

    A . Uniform Manifold Approximation and Projection (UMAP) of hippocampal transcriptome before and after one-way ANOVA thresholding by p<0.01 of the indicated genotype transcriptomes. B . PCA of transcriptomes after one-way ANOVA thresholding by p<0.01. Lines represent Euclidean distance clustering. C. Normalized hippocampal mRNA counts for Mrpl40 and Slc25a1 transcripts. Kruskal-Wallis test followed by Benjamini and Hochberg multiple corrections. D . Volcano plots and E . heat maps of paired comparisons between wild type hippocampi from single or transheterozygotic animals. Insets in volcano plots show percentage of diverse types of RNAs differentially expressed. Heat Maps depict z-scored hierarchical clustering of transcripts after thresholding with a cut-off fold of change=1.5 and p<0.01 (Welsh t test). F depict violin plots of Slc25a1 -/+ -sensitive mRNAs rescued in Slc25a1 -/+ ; Mrpl40 -/+ hippocampus. Kruskal-Wallis test followed by Benjamini and Hochberg multiple corrections. All panels wild type n=8 and all other genotypes n=7.

    Journal: bioRxiv

    Article Title: Suppressive Genetic Interactions Between Haploinsufficient Mitochondrial Genes Encoded in the 22q11.2 Microdeletion Locus Define Brain and Cardiac Phenotypes

    doi: 10.64898/2026.01.09.698677

    Figure Lengend Snippet: A . Uniform Manifold Approximation and Projection (UMAP) of hippocampal transcriptome before and after one-way ANOVA thresholding by p<0.01 of the indicated genotype transcriptomes. B . PCA of transcriptomes after one-way ANOVA thresholding by p<0.01. Lines represent Euclidean distance clustering. C. Normalized hippocampal mRNA counts for Mrpl40 and Slc25a1 transcripts. Kruskal-Wallis test followed by Benjamini and Hochberg multiple corrections. D . Volcano plots and E . heat maps of paired comparisons between wild type hippocampi from single or transheterozygotic animals. Insets in volcano plots show percentage of diverse types of RNAs differentially expressed. Heat Maps depict z-scored hierarchical clustering of transcripts after thresholding with a cut-off fold of change=1.5 and p<0.01 (Welsh t test). F depict violin plots of Slc25a1 -/+ -sensitive mRNAs rescued in Slc25a1 -/+ ; Mrpl40 -/+ hippocampus. Kruskal-Wallis test followed by Benjamini and Hochberg multiple corrections. All panels wild type n=8 and all other genotypes n=7.

    Article Snippet: To generate stable cell lines, SH-SY5Y cells (ATCC, CRL-2266; RRID:CVCL_0019) were transfected with ORF expression clone containing C terminally tagged Myc-DDK MRPL40 (Origene, RC202166) or N terminally tagged FLAG-SLC25A1 (GeneCopoeia, EX-A1932-Lv1020GS) as described .

    Techniques: